// Definition
A cheap, simple chromatography method using silica-coated plates and solvent baths to separate and visualize cannabinoid profiles from plant leaf extracts. Using Fast Blue B dye indicator, breeders can quickly identify Type I (THC), Type II (THC+CBD), and Type III (CBD) plants without expensive lab equipment.
// From the Episode
Ryan Lee uses TLC extensively in his breeding programs to screen hundreds of plants inexpensively. David Watson used it at Hortapharm. A plant ethanol extract is spotted onto the plate, developed in solvent, then stained to reveal distinct THC and CBD bands at different positions.