// Definition
A breeding technique where a female cannabis plant is pollinated with its own chemically-induced male pollen (using STS or ethephon to block ethylene). This limits the genetic experiment to only the two chromosome sets present in the original plant, making inheritance analysis simpler than sibling crosses where four parental chromosomes may be involved.
// From the Episode
Ryan Lee uses self crosses extensively to understand how traits segregate within a single genotype. The resulting S1 population shows F2-like segregation ratios. Key for creating feminized seed lines and analyzing terpene inheritance patterns.