// Definition
Cannabis has a uniquely challenging genome to sequence due to its high AT content (approximately 65%), diploid nature requiring sequencing of both maternal and paternal chromosomes, and the relative lack of focus on plant genetics compared to human genomics.
// From the Episode
Kevin McKernan from Medicinal Genomics discussed how cannabis genome sequencing was far more difficult than human genome sequencing. He explained the four DNA letters (ATCG) and how cannabis has about 65% AT content, making sequencing chemistry struggle. Every plant cell carries two genomes (mother and father), adding complexity. His team sequenced Chemdawg and put it in public databases, and also sequenced USL-31 and Finola with extensive RNA sequencing. Pacific Biosciences sequencers were emerging as better tools for this work.
// Source
Ep. 089 Hash Church LXXXVIII 88 →