// Definition
An advanced analytical chemistry technique combining hydrophilic interaction chromatography (HILIC) with reverse-phase chromatography (LCLC) to separate and identify complex, previously undetectable compounds in cannabis plant tissue, including trace flavo-alkaloids in leaves.
// From the Episode
Dr. Scialdone explained this cutting-edge method to the Hash Church audience. Traditional reverse-phase HPLC uses C18 (oleic acid) coated silica particles that interact with hydrophobic compounds like cannabinoids. HILIC uses hydrophilic interactions instead, allowing detection of water-soluble compounds. The two-dimensional approach combines both separation dimensions, enabling researchers to identify novel nitrogen-containing flavonoid glycosides that standard single-dimension methods would miss entirely.